IN CASE OF EMERGENCY:
Memory B cells rapidly produce Antibodies compared to Fire Stations rapidly produce First Responders
This paper explores how ridiculous it would be for communities to make logistical decisions about local fire departments based on a single assessment at one moment in time. Fire stations must be fully equipped and staffed at all times to be prepared to respond to any emergency situation. The average city with a population in excess of 10,000 people in the United States of America has 1.52 full time Fire Department employees for every 1000 population[1].
Fire Departments assess apparatus specification requirements including[2]:
- Primary function of the vehicles?
- Major functions required?
- How many riding positions are required?
- What is available funding?
- Will the new rig fit inside the firehouse?
In fact, managing the logistical needs of a Fire Department is one of the most important tasks to ensure operational readiness[3]. The National Fire Protection Association (NFPA) provides requirements for the number and training of firefighters that each department requires along with organizational structure to ensure efficient response to emergencies in every community[4].
The human Immune System must also be ready to respond to invading pathogens within moments of their detection. The foreign invader will set off alarms throughout the immune system at the cellular level responding through a variety of pathways capable of eliminating the attack. First responders including T cells, B cells, Natural Killer cells, antigen presenting cell, etc. working together to extinguish the threat. In the aftermath of the invasion the immune system will assess the damage taking appropriate measures ensuring the invader will not be able to wreak havoc on the human body as second time. This secondary response involves creating memory cells capable of responding with specific and deadly force eliminating the threat before it has a chance to replicate and cause damage.
One set of cells, the memory B cells, dominate in their ability to quickly respond upon re-exposure to a threat. The “efficiently trained” memory B cells only make antibodies to one specific antigen. When the antigen is re-encountered the memory B cells become antibody-secreting plasma cells (ASPC)[5]. Quiet memory B cells are ignited to become these antibody producing factories releasing hundreds to thousands of antibodies each second similar to firefighters responding to an alarm[6]. This phenomenal response is the reason vaccines work. Vaccines exploit natural immunity by exposing immune cells to an attenuated virus so when an active virus is encountered the threat will be quickly eliminated. Once the immune system has eliminated the threat the responding ASPC are no longer needed and they “stand down” and the amount of antibody in the body dissipates over time. Therefore, the amount of responding antibodies, or antibody titer, in a person’s blood varies depending on the current timeline of the person’s immune response. This is similar to how many first responders or firefighters are responding to an emergency, and how intense the emergency situation is followed by how long it takes to contain the hot spots and investigate the fire.
Whole blood of a healthy individual has about one million immune cells per milliliter. Spinning whole blood on a centrifuge forces the cells to the bottom and the predominantly water based plasma to the surface. Although the plasma does contain antibodies, the immune cells and therefore their responsiveness are removed. Using plasma based antibody testing to determine if an individual has immunity is similar to picking a point in time to count the firefighters, apparatus, and equipment currently responding to an emergency. The count might be 3 firefights getting a cat out of a tree, a dozen firefighters requiring 2 pumpers to put out a house fire, or maybe a massive three-alarm fire is occurring. Suggesting the outcome by measuring resources for only one single point in time that represents the abilities of the fire department is simply ridiculous. No communities evaluate the preparedness of their fire departments using such an asinine assessment. And yet this is what current antibody test do. What the individual paying for the antibody test really wants to know is if their immune cells will respond quickly to a specific invading pathogen. Quantifying antibodies at any given moment doesn’t prove or disprove immunity, and hence antibody test are considered to have a “high false negative” for assessing immunity. Is that what you want?
ImmunityAssessment.com is a unique worldwide science experiment simple enough for third graders to comprehend. This whole blood immunity assessment requires these simple steps:
- Draw two tubes of blood from a volunteer
- Tube 1 – place the tube on a rocker @37 C for 16-24hrs
- Tube 2 – add SARS-CoV-2 protein then place onto a rocket @37 C for 16-24hrs
- Tube 1 – add SARS-CoV-2 protein to the whole blood AFTER incubation
- Spin both tubes on the centrifuge to spin out the immune cells
- Plasma samples with tagged SARS-CoV-2 are added to ELISA plates coated with ACE-2[7]
- IF the viral proteins are able to bind with the host ACE-2 proteins the test is negative (-)
- IF the viral proteins are BLOCKED from binding ACE-2 by neutralizing antibodies (+)
- Use the ELISA plate reader to determine amount neutralizing antibodies in the sample
- Tube 1 – Is the amount of neutralizing antibodies present at time of blood draw
- Tube 2 – Is the amount of neutralizing antibodies AFTER incubation with viral proteins
An increase in the amount of neutralizing antibodies present AFTER inoculation with viral proteins indicates the individual produces a specific immune response within hours of exposure to SARS-CoV-2. This experiment combines assessing a volunteer’s whole blood for a rapid and specific immune response along with a questionnaire and access to all data.
Donors will be requested to:
- Cover the cost of adding their blood and questionnaire to the experiment (~$150)
- Donate at least 2 vials of blood for immunity assessment
- Spend the time to completely and honestly fill out the questionnaire.
- Did you have Covid-19? Dates? Severity scale 1-10?
- Did you receive a vaccine? Type? Dates? Boosters? Dates?
- Did you experience any adverse reactions to a vaccine? Dates? Severity scale 1-10?
- What medical health issues are you being treated for? Medications?
- Etc. etc. etc.…
This experiment provides one definite place to go for an up to date and comprehensive understanding of the pandemic crisis. We are asking groups, schools, unions, churches, fire departments, Governors, and individuals to join this mission to collect immunity assessment data. Collection of the data from numerous locations around the world is imperative to obtaining the current pulse of this pandemic. Individuals will be able to find out if their own blood responded within hours of being exposed to SARS-CoV-2 protein by producing neutralizing antibodies. No personal data will be stored and all scientific data will be made public and interactive. Anyone will be able to graph the data in numerous ways to answer questions such as:
- What is herd immunity for my area?
- % Vaccinated with greater than or equal to 60% increase in response upon SARS-CoV-2 exposure?
- % Unvaccinated with greater than or equal to 60% increase in response upon SARS-CoV-2 exposure?
- % Vaccinated reporting adverse reaction of greater than or equal to three percent
- % HIV+ with Covid-19 severity greater than or equal to eight percent
- Which has greater immunity – Vaccinated or Unvaccinated?
Please contact www.immunityassessment.com or www.ImmunoCure.org to find out more about setting up immunity assessment for your group. This is a simple ELISA plate experiment requiring minimal laboratory equipment, the cost of which will be recouped after the first 1000 volunteers donating to cover the cost of running their blood assessment. Yes, this means the lab setup plus the plates will require about $150,000. The plates are $990 and each will run 19-24 people plus standards (about $50/per)[8]. Think snow cone or hot dog cart not a fully functional scientific laboratory. The setup is minimal and your group owns everything. Unions wishing to fight mandates for their employees will have to keep records of the immunity assessment outcomes for each employee, as we will NOT be storing personal data for this experiment. All participants must understand this is NOT an FDA evaluated laboratory assessment and therefore the participating and covering the cost is strictly on a donation basis. Immunity Assessment.com will track the data and write explanations for the outcomes, but without FDA approval we will not be able to provide individual interpretations for any volunteers. We will fully explain how the experiment is done and volunteers will be able to make an informed interpretation of their blood assessment. We are only collecting data not providing an FDA approved laboratory test.
The labs will require:
- Sealed with proper air flow and HEPA filters
- Sterile hood
- Nonporous counter tops or covered porous materials
- CO2 incubators
- Centrifuge
- ELISA plate reader
- Refrigerator with freezer
- Pipettes / multi channel pipettes
- Blood rockers
- Needles / blood tubes
- Printer for barcode strips to identify blood tubes and barcode scanners
- Laboratory notebook software to record all data
- General supplies like pipette tips-chairs-computers
- Trained personnel to draw blood
- Trained lab personnel to inoculate the whole blood and run the ELISA plates
Once the lab is set up and the equipment is paid for, then your group will be able to raise money to pay for legal fees, support your unions, your church charities, etc. The ImmunoCure Foundation is using this as a fundraiser for our cancer research and we will be charging a fee for every entry into our database.
PLEASE JOIN US.
TAKE ACTION TO KEEP YOUR JOBS AND YOUR FREEDOMS.
WE ARE WORKING AS FAST AS WE CAN WITH LITTLE UPFRONT MONEY TO GET THIS GOING.
MAKE A DIFFERENCE. TAKE A STAND AGAINST MANDATES! FIGHT THEIR SO CALLED “SCIENCE” WITH REAL SCIENCE!
1. Staff, F.E., 1.52 Firemen per 1,000 Population. Fire Engineering.
2. Peters, W.C., Apparatus Specification Considerations: 10 Steps to Complete a Needs Assessment. The Rig, September 13, 2018.
3. Cain, D., The Need to Manage Logisitcs in the Fire Service. The Rig, 2016.
4. Security, K.F., NFPA Requirements for municipal and volunteer fire departments. 2021.
5. Nutt, S., Hodgkin, P, Tarlinton, D, The generation of anitbody-secreting plasma cells. Nature Reviews Immunology, 2015. 15: p. 160-171.
6. Hunter Allen, P.S., Histology, Plasma Cells. 2021.
7. GenScript. Available from: https://www.genscript.com/covid-19-detection-cpass.html.
8. Assessment, I. https://www.immunityassessment.com